Confidence Values
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Some annotations on VFB are based on predictions, for example, predicted neurotransmitters for neurons in electron microscopy datasets (see below). Where available, we include the confidence of these predictions (as a badge next to the annotation), as well as a link to the publication - in the example below, this would be by clicking on the ‘DOI’ badge.
Neurotransmitter Prediction Confidence Values
For MANC, optic-lobe and male-cns datasets, predictions are sourced from the neuprint predictedNt field, with data generated by Takemura et al. (2023), Nern et al. (2025) and Berg (2025), respectively. Predictions for FlyWire FAFB and Hemibrain datasets are conf_nt from Eckstein et al. (2024). BANC predictions are sourced from the Predicted NT type field of the Codex neurons.csv download and only used if not in conflict with the Verified NT type field, with data generated by Bates (2026).
In all cases, analysis was restricted to a particular set of neurotransmitters and assumed a single neurotransmitter per neuron (see publications for further detail). Neurons with fewer than 100 presynapses are excluded.
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