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L1 CNS (Larva1099) eFIB-SEM CATMAID Data Viewer

This CATMAID (Collaborative Annotation Toolkit for Massive Amounts of Image Data) instance hosts the enhanced focused-ion-beam SEM (eFIB-SEM) volume of a Drosophila first instar larva central nervous system (sample 1099), together with a curated set of identified, named neuron reconstructions.

Cross-section of the Larva1099 eFIB-SEM volume of the first instar larval CNS

Access

The Larva1099 CATMAID instance is hosted and maintained by Virtual Fly Brain (VFB) as part of its mission to integrate and preserve key Drosophila neuroscience datasets. The instance is accessible at: https://larva1099.catmaid.virtualflybrain.org/

This is a distinct volume from the L1EM serial-section TEM dataset also hosted by VFB: it is a separate first instar larva (sample 1099) imaged by enhanced focused-ion-beam scanning electron microscopy (eFIB-SEM) at 12 × 12 × 12 nm/voxel isotropic resolution.

Source Publication

The volume and reconstructions are from:

  • Randel N, Wang C, Clayton MS, Wang K, Pang S, Xu CS, Champion A, Hess HF, Cardona A, Keller PJ, Zlatic M (2026) Combining brain-wide activity imaging with electron microscopy reveals a distributed nociceptive network in the brain. bioRxiv 2025.09.25.678485. https://doi.org/10.1101/2025.09.25.678485 (preprint, in revision)

Dataset Contents

The viewer provides access to:

  • The complete eFIB-SEM image volume of a Drosophila first instar larva CNS (sample 1099), 12 × 12 × 12 nm/voxel, served as N5
  • A curated set of identified, named neuron reconstructions exported from the source project for hosting on VFB
  • The associated neuron and annotation metadata

The hosted set is the curated, named-neuron export rather than the complete connectome; each skeleton carries its neuron-name annotations.

Features

This CATMAID instance enables:

Public API token

Read-only access to this instance is open to everyone, but most of CATMAID’s query endpoints are POST rather than GET, and POST requests are subject to a CSRF check. Command-line and server-side clients (pymaid, curl, scripts) can satisfy that check by requesting a page first and replaying the cookie they are given. Code running in a browser cannot: it can neither read a cookie belonging to another site nor set a Referer header. Browser-based tools should therefore authenticate as the anonymous user, using this token:

b0cbcf16d84f2b820673471445e3d64d04797d06

This token is published deliberately and is not a secret. It authenticates as AnonymousUser, whose only permission on this project is can_browse, so it grants exactly the read access this page already offers to everyone and nothing further. Write requests made with it are refused by the server.

Send it in either the X-Authorization or the Authorization header:

curl -X POST https://larva1099.catmaid.virtualflybrain.org/1/skeleton/neuronnames \
  -H "X-Authorization: Token b0cbcf16d84f2b820673471445e3d64d04797d06" \
  --data "skids[0]=16"

Citation Guidelines

When using this data, please cite:

  1. The source dataset: Randel N, et al. (2026) Combining brain-wide activity imaging with electron microscopy reveals a distributed nociceptive network in the brain. bioRxiv 2025.09.25.678485. https://doi.org/10.1101/2025.09.25.678485

  2. The CATMAID platform: Saalfeld S, Cardona A, Hartenstein V, Tomančák P (2009) CATMAID: collaborative annotation toolkit for massive amounts of image data. Bioinformatics 25(15): 1984-1986. https://doi.org/10.1093/bioinformatics/btp266

Maintenance & Support

This resource is archived, hosted, and maintained by Virtual Fly Brain (VFB - https://virtualflybrain.org) as part of its commitment to preserving and making accessible critical Drosophila neuroscience data resources.