third instar - cleared gut stage [FBdv_00007021]

The substage of the wandering third instar larval stage when the larva has cleared its gut contents. Temporal ordering number - 467. This is commonly assayed by adding 0.5% bromophenol blue to the food and looking for wandering third instar larvae with guts lacking any blue stain (Andres and Thummel, 1994). Larvae at this stage are mainly in chromosomal puff stages 7-9 and pupate within 1-6 hours (Andres and Thummel, 1994.
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Open third instar - cleared gut stage in VFB

VFB Term Json

{
    "term": {
        "core": {
            "iri": "http://purl.obolibrary.org/obo/FBdv_00007021",
            "symbol": "",
            "types": [
                "Entity",
                "Class"
            ],
            "short_form": "FBdv_00007021",
            "label": "third instar - cleared gut stage"
        },
        "description": [
            "The substage of the wandering third instar larval stage when the larva has cleared its gut contents."
        ],
        "comment": [
            "Temporal ordering number - 467. This is commonly assayed by adding 0.5% bromophenol blue to the food and looking for wandering third instar larvae with guts lacking any blue stain (Andres and Thummel, 1994). Larvae at this stage are mainly in chromosomal puff stages 7-9 and pupate within 1-6 hours (Andres and Thummel, 1994."
        ]
    },
    "query": "Get JSON for Class",
    "version": "44725ae",
    "parents": [
        {
            "symbol": "",
            "iri": "http://purl.obolibrary.org/obo/FBdv_00005259",
            "types": [
                "Entity",
                "Class",
                "preferred_root"
            ],
            "short_form": "FBdv_00005259",
            "label": "developmental stage"
        }
    ],
    "relationships": [
        {
            "relation": {
                "iri": "http://purl.obolibrary.org/obo/FBdv_00018001",
                "label": "substage_of",
                "type": "substage_of"
            },
            "object": {
                "symbol": "",
                "iri": "http://purl.obolibrary.org/obo/FBdv_00005341",
                "types": [
                    "Entity",
                    "Class"
                ],
                "short_form": "FBdv_00005341",
                "label": "wandering third instar larval stage"
            }
        },
        {
            "relation": {
                "iri": "http://purl.obolibrary.org/obo/RO_0002087",
                "label": "immediately_preceded_by",
                "type": "immediately_preceded_by"
            },
            "object": {
                "symbol": "",
                "iri": "http://purl.obolibrary.org/obo/FBdv_00007020",
                "types": [
                    "Entity",
                    "Class"
                ],
                "short_form": "FBdv_00007020",
                "label": "third instar - partially cleared gut stage"
            }
        }
    ],
    "xrefs": [],
    "anatomy_channel_image": [],
    "pub_syn": [],
    "def_pubs": [
        {
            "core": {
                "symbol": "",
                "iri": "http://flybase.org/reports/FBrf0076598",
                "types": [
                    "Entity",
                    "Individual",
                    "pub"
                ],
                "short_form": "FBrf0076598",
                "label": "Andres and Thummel, 1994, Goldstein, Fyrberg, 1994: 565--573"
            },
            "FlyBase": "",
            "PubMed": "7535884",
            "DOI": ""
        }
    ]
}