L5 [FBbt_00003725]

A lamina monopolar neuron that hardly arborizes at all in the lamina, but forms a branched arborization in medulla layers M1 and M2 and a clump of terminals in M5. In the medulla, it forms strong reciprocal synaptic connections with monopolar lamina monopolar neuron L1 (Takemura et al., 2013. It also receives substantial input from L2 and C2 neurons and has many presynapses connecting to medullary intrinsic neurons Mi1 and Mi4, and to transmedullary neuron Tm3a (Takemura et al., 2013. It is a cholinergic neuron (Davis et al., 2020. There is usually one of these cells per optic column (Nern et al., 2025. (Fischbach and Dittrich, 1989, Rivera-Alba et al., 2011, Meinertzhagen and O’Neil, 1991, Takemura et al., 2013, Takemura et al., 2008, Davis et al., 2020)

Tags:

L5

ID: FBbt_00003725

Adult Cholinergic Nervous system Visual system

A lamina monopolar neuron that hardly arborizes at all in the lamina, but forms a branched arborization in medulla layers M1 and M2 and a clump of terminals in M5. In the medulla, it forms strong reciprocal synaptic connections with monopolar lamina monopolar neuron L1 (Takemura et al., 2013. It also receives substantial input from L2 and C2 neurons and has many presynapses connecting to medullary intrinsic neurons Mi1 and Mi4, and to transmedullary neuron Tm3a (Takemura et al., 2013. It is a cholinergic neuron (Davis et al., 2020. There is usually one of these cells per optic column (Nern et al., 2025. (Fischbach and Dittrich, 1989, Rivera-Alba et al., 2011, Meinertzhagen and O'Neil, 1991, Takemura et al., 2013, Takemura et al., 2008, Davis et al., 2020)

Pre- versus postsynaptic innervation judged by scoring of terminal morphology from figures in Fischbach and Dittrich, 1989 as assessed by FlyBrain Neuron DB. Connectivity in the medulla was extrapolated from serial EM of 7 columns from a single sample (Takemura et al., 2013.

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Classification

Relationships

develops from: immature lamina monopolar neuron L5
has synaptic IO in region: medulla layer M2
receives synaptic input from neuron: centrifugal neuron C2, lamina monopolar neuron L1, lamina monopolar neuron L2
receives synaptic input in region: lamina, medulla layer M1, medulla layer M5
sends synaptic output to cell: lamina monopolar neuron L1, medulla intrinsic neuron Mi1, medulla intrinsic neuron Mi4, transmedullary neuron Tm3
sends synaptic output to region: medulla layer M1, medulla layer M5

Alternative Names

Synonym	Scope	Reference
L5	related synonym	Berg et al., 2025, Bates et al., 2025, Nern et al., 2025, name_in_male-cns, Schlegel et al., 2024
monopolar laminar cell L5	exact synonym
L5	exact synonym	Özel et al., 2021

Cross References

L5 on FlyBrain NeuronDB (10093)

Downloads

Image files aligned to L5_L (MaleCNS:536013):

Image files aligned to L5_L (MaleCNS:179312):

Image files aligned to L5_L (MaleCNS:519149):

Image files aligned to L5:

Image files aligned to C2:

Image files aligned to L5:

Image files aligned to L5_R (JRC_OpticLobe:97491):

Image files aligned to L5_L (MaleCNS:179312):

List all available images of L5 (7306 total)

Thumbnail	Name	Tags
	L5_L (MaleCNS:103756)	Nervous_system, Adult, Visual_system, Cholinergic
	L5_L (MaleCNS:591864)	Nervous_system, Visual_system, Adult, Cholinergic
	L5_R (MaleCNS:104308)	Nervous_system, Visual_system, Adult, Cholinergic
	L5_R (MaleCNS:107142)	Nervous_system, Adult, Visual_system, Cholinergic
	L5_R (MaleCNS:111118)	Nervous_system, Visual_system, Adult, Cholinergic

View all 7306 results in VFB →

Splits targeting L5 (2 total)

Thumbnail	Name	Tags
	P{R31H09-GAL4.DBD} ∩ P{R21A05-p65.AD} expression pattern	Split
	P{R37E10-GAL4.DBD} ∩ P{R64B07-p65.AD} expression pattern	Split

View all 2 results in VFB →

scRNAseq data for L5 (23 total)

Thumbnail	Name	Tags
	scRNAseq_2020_Kurmangaliyev_MAIN_seq_clustering_L5_neurons	Adult, Cluster, Nervous_system
	scRNAseq_2021_Ozel_ADM1_seq_clustering_L5_neurons	Adult, Cluster, Nervous_system
	scRNAseq_2022_FCA_MALE_FULL_seq_clustering_L5_neurons	Adult, Cluster, Nervous_system
	scRNAseq_2022_FCA_FEMALE_FULL_seq_clustering_L5_neurons	Adult, Cluster, Nervous_system
	scRNAseq_2022_FCA_FEMALE_HEAD_seq_clustering_L5_neurons	Adult, Cluster, Nervous_system